Definition Of Growth Promotion Test

Following pharmacopeia guidelines labs perform growth promotion tests to determine the suitability of culture media used for microbial enumeration tests sterility tests and tests for specified microorganisms.

Definition of growth promotion test. But before using that media in any testing media must qualify gpt. Scope this sop is applicable for media growth promotion inhibition and sterility check in microbiology laboratory of pharmaceutical company name. Use non selective agar as a control when testing liquid media. Responsibility chemist or above of qc laboratory.

Growth promotion test is used for the qualification of the media we use different media for microbiology testing. The purpose of the growth promotion test is to ensure the media used to test products can recover small numbers of organisms testing in parallel eliminates all variables except for the media use best practices to obtain accurate and consistent results. Read our 9 tips for growth promotion testing on selective media for more on this topic. The growth promotion test may also be known as nutritional adequacy testing fertility testing media challenge testing or quality control testing of media.

5 3 growth promotion test of sterilized media. Throughout this article the term growth promotion test will be used and it encompasses the associated tests such as sterility checks ph checks inhibitory assays and indicative assays. Recovery specification applied is 50 200 recovery when the new batch test is compared to a previously certified batch control. In growth promotion test media are inoculated with different kinds of microorganisms as mentioned in pharmacopoeia.

To determine if a new batch of liquid media is acceptable test the new batch of media in parallel with a previously approved batch of media and non selective agar. 5 3 1 select the quantified microbial culture as per annexure i for the growth promotion test 5 3 2 quantified culture should be 24 hours old or a validated period old microbial culture can be used for growth promotion having count 10 100 cfu ml. Sop on media growth promotion inhibition and sterility check objective to lay down the procedure for media growth promotion inhibition and sterility check. Gpt should be performed on solid media and broths but not for dilution solutions and rinsing fluids.

Quantitative analysis used for media that is used for enumeration testing i e. Growth promotion testing consists of three types of tests. Tryptone soya agar place count agar etc.